Ultrasonic homogenizer in Nano materials field attracted the attention of scientists, researchers and engineers of almost any branches as nano-sized particles show unique characteristics. But the smaller particles, the more difficult becomes treatment. Ultrasonic homogenizer is often the best method to effect nano particles effectively. Mostly, high power ultrasonic homogenizers are the only efficient way to achieve the desired milling and dispersing results of nano particles such as nanotubes, graphene, metal oxides etc.
The impact of sonication on biological and microbiological systems are manifold: Dispersing & Homogenizing, cell and tissue lysis (e.g. bacteria, yeast, viruses, algae…) & extraction of intracellular materials such as proteins, ribosomes, RNA, lipids, peptides…, plant cell transformation.
The homogenizer is also used for numerous application in the food, nutritional supplement and pharmaceutical industry to release bioactive compounds such as vitamins, polyphenols, polysaccharides, cannabinoids and other phytochemicals from botanicals, including extraction, homogenization, pasteurization and fermentation. As a non-thermal treatment, ultrasonication improves production processes by higher yields, higher quality as well as time- and cost-saving processing.

Graphene is a two-dimensional carbon nanomaterial composed of carbon atoms with sp⊃2; hybrid orbitals forming a hexagonal honeycomb lattice. Graphene is a carbon-atom-thick sheet of carbon that forms graphite through non-bonded interactions and has an extremely large surface area. Ultrasonic homogenizer uses the cavitation effect of ultrasonic waves to disperse agglomerated particles. It puts the particle suspension to be processed into the sound field and processes it with appropriate ultrasonic amplitude. Under additional effects such as cavitation effect, high temperature, high pressure, micro-jet, strong vibration, etc., the distance between molecules will continue to increase, eventually causing the molecules to break and form a single molecular structure. This product is particularly effective in dispersing nanomaterials (such as carbon nanotubes, graphene, silica, etc.). Because of the chemical nature of carbon the dispersing behavior in water is rather difficult. As shown in the video, it can be easily demonstrated that ultrasonication is capable to disperse graphene effectively.

Gingerol has a strong anti-lipofuscin effect. In daily production, gingerol is generally extracted by oil extraction method. The production steps are complicated, time-consuming and the extraction rate is low. In order to improve the efficiency, ultrasonic extraction was carried out, the solid-liquid ratio was 1:16, the ultrasonic treatment temperature was 60 degrees Celsius, the treatment time was 30 minutes, the optimal extraction concentration could reach 16.1ug/ml, and the extraction rate reached 35.8%.

Mushroom polysaccharides can improve human immunity, reduce blood sugar, blood lipids, cholesterol, improve diabetes and arteriosclerosis. The common hot water reflux extraction method is to soften and break the plant cell wall by continuous heating, so that the polysaccharide diffuses from inside the cell to outside the cell and then diffuses into the external solvent. The equipment is simple, the cost is low, but the process extraction interval is long. Ultrasonic extraction can make up for this shortcoming, using the cavitation effect to break plant cell wall, shorten the extraction time of active components, and low energy consumption, high extraction rate, environmental protection.

Conventional oil-water emulsification, using mechanical mixing method (high-speed shear pump, etc.), but the effect and stability lack, need to add a lot of stabilizer. However, with ultrasonic technology, the ultrasonic homogenizer outcome quality is high. The average droplet size of the formed emulsion is small, which can reach 0.2~2um. The droplet size distribution range is narrow, up to 0.1~10um. The concentration is high, the concentration of pure emulsion can reach 30%, adding emulsifier can reach 70%.
The emulsification is stable, you can produce stable emulsions without or with less emulsifiers, and some can be stable for several months or even more than half a year, with low energy consumption, high production efficiency and low cost.

In the field of essential oil extraction, ultrasonic extraction technology has been widely used. Taking rose essential oil as an example, the traditional distillation method requires a lot of water and energy, and takes a longer time, while ultrasonic extraction technology can extract more essential oil in a shorter time, which is more than twice the traditional method. The extraction temperature is reduced by 20~30℃, which is conducive to the extraction of heat-sensitive chemical components and reducing energy consumption. In addition, ultrasonic extraction technology can also improve the quality of essential oils, making them more pure and more fragrant.

Radix paeoniae alba has the functions of tonifying blood, relieving pain, improving human immunity and expanding. Due to the temperature, the loss rate of paeoniflorin in traditional water decocting process is as high as 40%. The extraction liquid of ultrasonic extraction process has higher clarity, less magazine content, simple processing process and short extraction time. Moreover, it can solve the effect of high temperature and improve the extraction rate. Under the conditions of solid-liquid ratio 1:40, time 35 minutes and particle size 100, the dissolution amount of Paeoniflorin in Paeoniflorin was the highest. Take a look at the following video showing the ultrasonic extraction effect.

The homogenizer is mainly through mechanical shear, vibration and pressure, physical treatment of the material, so that the particle distribution is uniform, to achieve the purpose of homogenization. The high-pressure homogenizer uses a high-pressure filter membrane to compress the material under high pressure to form a high-speed fluid form. The second ultrasonic homogenizer uses the cavitation effect of ultrasonic waves to disperse, break and homogenize the material.

 Ultrasonic homogenizer produce small particle/droplet and narrow distribution curves, it can reach the nanoscale, which is of great help for laboratory research of Nano materials field. 

Ultrasonic homogenizers exhibit exceptional performance and applicability in high-load, large-scale applications. Their superior capabilities shine when handling complex tasks and processing substantial sample volumes. With precision and efficiency, these devices ensure uniform results in industrial settings. Whether disrupting cells in bioprocessing or dispersing particles in chemical manufacturing, ultrasonic homogenizers stand out for their versatility. Their remarkable efficiency in managing intricate processes and substantial sample sizes makes them a preferred choice for industries requiring reliable and high-throughput solutions.

Sample Type: E. coli bacterial cultures
Processing Volume: 500 liters per batch
Expected Performance:
Cell Disruption Efficiency: Achieving over 90% disruption for optimal release of intracellular components.
Protein Extraction: Efficient extraction of recombinant proteins with yields exceeding 95%.

Sample Type: Polyethylene terephthalate (PET) resin
Processing Volume: 1,000 kilograms per batch
Expected Performance:
Particle Size Reduction: Consistent reduction of particle size to below 5 microns for improved material properties.
Homogeneous Dispersion: Achieving uniform dispersion of additives for enhanced product quality.

Ultrasonic homogenizers showcase exceptional flexibility across diverse laboratory and industrial applications. In laboratory settings, these versatile devices adapt seamlessly to varying scales. For instance, in small-scale experiments, they proficiently process microliter to milliliter-sized samples with precise frequency adjustments, catering to the specific needs of researchers. Larger-scale laboratory applications witness ultrasonic homogenizers handling volumes in the range of liters, demonstrating their adaptability to increased sample sizes. The frequency can be adjusted accordingly to meet the demands of different experimental setups. This flexibility makes ultrasonic homogenizers indispensable tools, offering tailored solutions for a broad spectrum of applications, regardless of the scale or complexity of the experiment.

High-speed homogenizer offer distinct advantages in high-performance experiments and applications, demonstrating exceptional efficacy in demanding environments. Notable features contributing to their success include:

High-performance experiments aiming to enhance cell disruption efficiency using ultrasonic homogenizers, specific requirements include.

Requirement: Precise tuning of ultrasonic frequency to match the cell type for optimal disruption efficiency.
Rationale: Different cell types respond differently to ultrasonic frequencies; customization ensures effective disruption without compromising cell viability.

Requirement: Ability to control amplitude settings for fine-tuning energy delivery to the sample.
Rationale: Achieving controlled disruption without excessive heat generation is crucial, ensuring the integrity of intracellular components.

Requirement: Incorporation of monitoring systems for real-time feedback on sample integrity during homogenization.
Rationale: Ensures immediate adjustments if needed, preventing over-processing or under-processing of the cells.

Requirement: Integration of a cooling system to manage heat generated during cell disruption.
Rationale: Prevents thermal damage to sensitive cellular components and maintains the overall health of the sample.

Requirement: Versatile probe designs suitable for different sample volumes and types.
Rationale: Enables researchers to process various sample sizes with optimal efficiency, promoting flexibility in experimental design.

Requirement: Implementation of methods to assess cell viability post-homogenization.
Rationale: Ensures that the disruption process does not compromise the overall viability of cells, especially crucial for downstream applications.

Requirement: Scalable design to accommodate high-throughput processing requirements.
Rationale: Enables consistent and efficient cell disruption across multiple samples simultaneously, crucial for large-scale experiments and industrial applications.

Meeting these specific requirements ensures that ultrasonic homogenizers perform optimally in enhancing cell disruption efficiency, providing reliable and reproducible results in high-performance experiments.